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Add 2 steel balls and 1 ml TCA 5% per tube. Close tube rmly and wrap with Para lm. Disintegrate scaffold by using a MinibeadBeater 3 times at 25,000 rpm for 10 s each time. Place on ice between cycles for cooling. Incubate the tubes at room temperature for 30 min. Centrifuge at 3000 g for 10 min. Pipette the supernate into a clean, labeled tube without disrupting the pellet. Add 1 ml of TCA 5% into the tube with the steel balls. Incubate the tubes at room temperature for 30 min. Combine the 2 corresponding TCA solutions. Centrifuge at 3000 g for 10 min. Transfer supernate into a clean, labeled tube without disrupting the pellet.

[1] 3GPP: Generic Authentication Architecture (GAA); Generic Bootstrapping Architecture Technical Speci cation 33220 Online: www3gpporg/ftp/Specs/html-info/33220htm [2] Ardissono L, Goy A, Petrone G, Segnan M and Torasso P: INTRIGUE: personalized recommendation of tourist attractions for desktop and handset devices Applied Arti cial Intelligence, Special Issue on Arti cial Intelligence for Cultural Heritage and Digital Libraries, Vol 17, No 8/9, pp 687 714 Taylor & Francis, 2003 [3] Conaty G (ed): Initial Scenarios, Requirements and Guidelines: User-centred approach for the design of future mobile services and applications IST-MobiLife Project Deliverable D6b (D11b), February 2006 Online: wwwist-mobilifeorg [4] Cranor LF, Reagle J and Ackerman MS: Beyond Concern: Understanding net users attitudes about online privacy Technical Report TR 9943, AT&T Labs-Research, 1999 Online: wwwresearchattcom/ library/trs/TRs/99/994/ [5] Cranor, LF and Reagle J.

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Illustrated TCP/IP by Matthew G. Naugle Wiley Computer Publishing, John Wiley & Sons, Inc. ISBN: 0471196568 Pub Date: 11/01/98

: Designing a Social Protocol: Lessons learned from the platform for privacy preferences In MacKie-Mason JK and Waterman D (eds), Telephony, the Internet, and the Media Mahwah, NJ: Lawrence Erlbaum Associates, 1998 Online: wwwresearchattcom/ lorrie/pubs/dsp/ [6] Dodgeball: Online: wwwdodgeballcom [7] Erickson T and Kellogg WA: Social Translucence: an approach to designing systems that support social processes ACM Transactions on Computer Human Interaction, Vol 7, No 1, 2000, pp 59 83 [8] Fruchterman TMJ and Reingold EM: Graph Drawing by Force-directed Placement Software Practice and Experience, Vol 21, No 11, 1991, pp 1129 1164 [9] Grandison T and Sloman M: A Survey of Trust in Internet Applications IEEE Communications and Survey, Vol 3, No 4, 2000, pp 2 16 [10] Iachello G, Smith I, Consolvo S, Chen M and Abowd G.

Entropy of encoded bits. Let C : X {0, 1} be a nonsingular but nonuniquely decodable code. Let X have entropy H (X). (a) Compare H (C(X)) to H (X). (b) Compare H (C(X n )) to H (X n ). Code rate. Let X be a random and distribution 1 with X= 2 with 3 with variable with alphabet {1, 2, 3} probability probability probability

: Developing Privacy Guidelines for Social Location Disclosure Applications and Services ACM International Conference Proceeding Series, Vol 93 Proceedings of the 2005 symposium on Usable privacy and security (SOUPS) [11] Internet Engineering Task Force (IETF): Group Key Management Protocol (GKMP) Architecture RFC 2094 Online: wwwfaqsorg/rfcs/rfc2094html [12] Internet Engineering Task Force (IETF): Group Key Management Protocol (GKMP) Speci cation RFC 2093 Online: wwwfaqsorg/rfcs/rfc2093html [13] Internet Engineering Task Force (IETF): GSAKMP: Group Secure Association Key Management Protocol RFC 4535 Online: wwwfaqsorg/rfcs/rfc4535html [14] Internet Engineering Task Force (IETF): Internet Key Exchange (IKEv2) Protocol RFC4306 Online: wwwfaqsorg/rfcs/rfc4306html [15] Internet Engineering Task Force (IETF): Internet Security Association and Key Management Protocol (ISAKMP) RFC2408 Online: wwwfaqsorg/rfcs/rfc2408html [16] Internet Engineering Task Force (IETF): Multimedia Internet Keying (MIKEY) RFC3830 Online: wwwfaqsorg/rfcs/rfc3830html.

[17] Internet Engineering Task Force (IETF): The Internet Key Exchange (IKE) RFC2409 Online: wwwfaqsorg/rfcs/rfc2409html [18] Johnson DW and Johnson FP: Joining Together: Group theory and group skills, 3rd edn Englewood Cliffs, NJ: Prentice-Hall, 1987 [19] Kurvinen E (ed): Results of Service and Application Evaluation IST-MobiLife Project Deliverable D14 (D19), December 2006 Online: wwwist-mobilifeorg [20] Masthoff J: Group Modeling: Selecting a sequence of television items to suit a group of viewers User Modeling and User-Adapted Interaction, Vol 14, 2004, pp 37 85..

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Figure 8-1

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